Études sur le cancer du sein - Studien zu Brustkrebs - Breast Cancer Studies

ImunoBran® = BioBran®

Modified Arabinoxylan Rice Bran (MGN-3/Biobran) Enhances Yeast-induced Apoptosis in Human Breast Cancer Cells In Vitro                                                                  PEER REVIEWED

  • M. Ghoneum and S. Gollapudi (edited by Chris Gutch PhD.)

We have recently reported that phagocytosis of killed Saccharomyces cerevisiae, baker's yeast, induced apoptosis in human breast cancer cell lines MCF-Z, ZR- 75-1 and HCC70. In this study we have evaluated the ejfect of treatment with MGN-3, a modified arabinoxylan from rice bran, on phagocytosis and yeast-induced apoptosis in breast cancer cells. Cancer cells were cultured with yeast at a ratio of 1:10 in the absence or presence of MGN-3, and the percentages of phagocytic and apoptotic cancer cells were examined by flow cytometry and by cytospin preparations. Cancer cells treated with MGN-3 exhibited increased percentages of attachment (200%) and uptake of yeast (313%) by MCF-7 cells at 0.5 hr, as compared with cells without MGN-3. In addition, treatment with MGN-3 resulted in a 2 fold increase in the percentage of apoptotic MCF-7 cells, 2.5 fold for ZR-75 cells and 1.8 fold for HCC70 cells. MGN-3 effect was dose-dependent and associated with increased activation of caspases 8 and 9 in MCF-7 cells, and caspases 8, 9 and 3 in HCC70 cells. This data demonstrates that MGN-3 accelerates phagocytosis-induced apoptosis of cancer cells, which may represent a novel therapeutic strategy for the treatment of breast cancer.

L'arabinoxylane modifié au son de riz, le MGN-3/ImunoBran, sensibilise les cellules métastastiques du carcinome du sein au paclitaxel in vitro 

  • MAMDOOH GHONEUM1, NARIMAN K. BADR EL-DIN2, DOAA A. ALI2 et MAI ALAA EL-DEIN2 1

Chaire d'othorhinolaryngologie, Université Drew de Médicine et des Sciences, Los Angeles, CA, U.S.A.; 2Chaire de zoologie, Faculté des sciences naturelles, Université de Mansoura, Mansoura, Égypte 

Extrait - Arrière-plan : Il existe un intérêt grandissant pour les méthodes alternatives de traitement visant la réduction de la toxicité de la chimiothérapie par la diminution de la concentration des agents chimio thérapeutiques tout en conservant l'intensité actuelle des effets de ces derniers sur les cellules cancéreuses. Les études précédentes ont démontré que l'arabinoxylane de son de riz, le MGN-3/ImunoBran, sensibilisait les cellules tumorales mammaires humaines (BCC) à la daunorubicine (DNR). Dans l'étude présente, nous avons fait l'évaluation de la capacité du produit MGN-3 à sensibiliser les cellules à d'autres types d'agents chimiothérapeutiques. Matériel et méthodes : cellules non métastatiques MCF-7 (BCC humaines) et cellules métastatiques 4T1 (BCC de souris) ont été cultivées avec diverses concentrations de paclitaxel en présence et en absence de MGN-3. On a examiné la survie des cellules, l'endommagement de l'ADN et la prolifération des cellules. Résultats : le MGN-3 a augmenté la sensibilité des deux types de cellules cancéreuses au paclitaxel de plus de 100 fois. Du point de vue mécanique, le MGN-3 fonctionne de manière synergique avec le paclitaxel : il endommage l'ADN, améliore l'apoptose et inhibe la prolifération des cellules AT1. Conclusion : nos données prouvent que le MGN-3 est un sensibilisateur chimique efficace et peut représenter un complément nouveau du traitement du cancer métastasé du sein.

Effect of reducing side effects of chemotherapy in breast cancer patients

  • Masood AI, Sheikh R, Anwer RA (edited by Chris Gutch PhD.) 2013

Objective: The aim of study was to assess the effect of Biobran in reducing of chemotherapy induced side effects in terms of tiredness, anorexia, vomiting and hair loss and quality of life in terms of weight loss.

Setting: Radiotherapy Department, Nishtar Hospital Multan.

Material and Methods: Fifty patients of breast cancer were enrolled randomly in two groups. Group-A patients were given 3 gram dose of Biobran MGN-3 per day one week before and one week after chemotherapy. Group-B patient were given chemotherapy alone. Total six cycles of chemotherapy were given. No multivitamin orfood supplements were given during this study.

Chemotherapy induced side effects (tiredness, anorexia, and vomiting, hair loss) were assessed by questionnaire to the patients before start of each cycle. Weight was checked before each cycle to assess weight gain or loss. White blood cells were checked by complete blood count just before and one week after chemotherapy.

Results: Between six months, 50 patients were enrolled in Radiotherapy Department, Nishtar Hospital Multan. There was asignificant reduction in tiredness and anorexia in group-A patients. 20 (80%) patients of group-A felt increase intheirdiet and notiredness without any appetizeror multivitamin. But group-B patients demanded for appetizer due to severe anorexia after chemotherapy except 3 (12%) patients who didn't use any appetizer or food supplement. In group-A, 15 (60%) patients didnt need any anti-emetic as compared to group-B all patient (100%) experienced severe nausea during and after chemotherapy. Group-A patients experienced less hairfall 7 (28%) patients as compared to other group which is 25 (100%) patients.

Conclusions: The study showed that, by helping to optimize the immune system, Biobran MGN-3 can not only help maximize treatment success, but also minimize treatment side effects and improve quality of life during treatment and in recovery.

Modified arabinoxylan from rice bran, sensitizes human breast cancer cells to chemotherapeutic agent, daunorubicin                                                           PEER REVIEWED

  • S. Gollapudi and M. Ghoneum (edited by Chris Gutch PhD.) 2008

MGN-3/Biobran, a modified form of arabinoxylan from rice bran, is a potent biological response modifier (BRM). Our previous studies demonstrated that MGN-3 sensitizes human leukemia cells to death receptor [CD95]-induced apoptosis [Ghoneum M, Gollapudi S. MGN-3 sen sitizes human T cell leukemia cells to death receptor (CD95)-induced apoptosis. Cancer Lett 2003;20l :41-9]. In this study, we evaluated the chemo-sensitizing activity of MGN-3 against human breast cancer cells (BCCs) in vitro. Methods: BCCs (MCF-7 and HCC70 cells) were cultured with different concentrations of daunorubicin (DNR) (from 1 x10⁻⁹ to 1×10⁻⁶M) in the presence or absence of selected concentrations of MGN-3 (100-1000 µg/ml) for 3 days. Cancer cell survival was determined by MTT assay and drug accumulation was determined by flow cytometry. Results: Treatment with MGN-3 increased susceptibility of BCCs to DNR (5.5 -fold for MCF-7 and 2.5-fold for HCC70 cells) as compared to BCCs treated with DNR alone. The sensitizing effect of MGN-3 was associated with increased accumulation of DNR in cancer cells. Conclusions: Our data demonstrate that MGN-3 is an effective chemo-sensitizer and may represent a potential novel adjuvant for the treatment of breast cancer.

Chemopreventive Properties of Dietary Rice Bran: Current Status and Future Prospects 

  • Angela J. Henderson,3 Cadie A. Ollila,3 Ajay Kumar,3 Erica C. Borresen,3 Komal Raina,4 Rajesh Agarwal,4,5 and Elizabeth P. Ryan3,5* 3 Department of Clinical Sciences, Colorado State University, Fort Collins, CO; 4 Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anshutz Medical Campus, Aurora, CO; and 5 University of Colorado Cancer Center, University of Colorado, Aurora, CO 

ABSTRACT Emerging evidence suggests that dietary rice bran may exert beneficial effects against several types of cancer, such as breast, lung, liver, and colorectal cancer. The chemopreventive potential has been related to the bioactive phytochemicals present in the bran portion of the rice such as ferulic acid, tricin, b-sitosterol, g-oryzanol, tocotrienols/tocopherols, and phytic acid. Studies have shown that the anticancer effects of the rice bran-derived bioactive components are mediated through their ability to induce apoptosis, inhibit cell proliferation, and alter cell cycle progression in malignant cells. Rice bran bioactive components protect against tissue damage through the scavenging of free radicals and the blocking of chronic inflammatory responses. Rice bran phytochemicals have also been shown to activate anticancer immune responses as well as affecting the colonic tumor microenvironment in favor of enhanced colorectal cancer chemoprevention. This is accomplished through the modulation of gut microflora communities and the regulation of carcinogen-metabolizing enzymes. In addition, the low cost of rice production and the accessibility of rice bran make it an appealing candidate for global dietary chemoprevention. Therefore, the establishment of dietary rice bran as a practical food-derived chemopreventive agent has the potential to have a significant impact on cancer prevention for the global population. Adv. Nutr. 3: 643-653, 2012.

Modified Rice Bran Beneficial for Weight Loss of Mice as a Major and Acute Adverse Effect of Cisplatin

  • Yuzo Endo, Hiroshi Kanbayashi (edited by Chris Gutch PhD.) 2003

Alkylating agents and antimetabolites remain prominent in a wide variety of cancer chemotherapy protocols on the basis of more selective effects on the faster than normal mitotic cycles of malignant cells. Among new platinum‐containing anticancer molecules, the lead compound cisplatin (Cis‐platinum (II) diammine dichloride) has been known to possess beneficial anticancer properties in terms of specific interaction of cisplatin and DNA strands. Cisplatin (Rosenberg et al. 1969) has been encouraged for treatment of head and neck, bladder, and cervical cancers (Loehrer & Einhorn 1984), as well as breast cancer (Smith & Talbot 1992). Although the prevalent incidence of lung, gastric, breast, colorectal and prostate cancers place them in top ranks of the most common cancers in the civilized countries, many of these advanced cancers are unresponsive to chemotherapy. Platinum‐based drugs would be a potent choice in these situations, but they frequently cause substantial side effects, such as nausea, vomiting, nephropathy and hypomagnesaemia due to damage of renal tubules (Lajer & Daugaard 1999). Furthermore, in addition to hearing loss and peripheral neuropathy, myelosuppression is one of the most devastating suppressive side‐effects (Prestayko et al. 1979) leading to immunocompromized states. Therefore, any reduction of the side effects of cisplatin would be valuable. Thus, we explored the effect of modified rice bran on protection of weight loss of mice under tolerable maximal doses of cisplatin. Others have briefly commented on the antistress and antifatigue effects of fermented rice bran (Kim et al. 2001) or some beneficial effect of modified rice bran on some adverse actions of anticancer drugs in rats (Jacoby et al. 2000).

The orally administration of the Hydrolysis Rice Bran prevents a common cold syndrome for the elderly people based on immunomodulatory function

  • Kenji Tazawa, Kenichi Ichihashi, Takako Fujii, Kazunobu Omura, Mari Anazawa, Hiroaki Maeda (edited by Chris Gutch PhD.) 2003

The preventive effect of RIBEX against the common cold syndrome was examined on elderly people. RIBEX, containing arabinoxylan derivatives Hydrolysis Rice Bran (HRB), was prepared from the water soluble dietary fiber fraction from rice bran through partial processing by carbohydrate complex of Lentinus Edodes mycelia (shiitake). Using the non-chemical and non-biological treated water soluble fraction of Rice Bran (RB) as control, the examination was conducted by the cross-over double blind manner through the administration term of 6 weeks for each material. Fifty elderly people who stayed in a care institution under Japan`s Long-term Care Insurance participated in this study. The results of thirty-six participants who showed comparative data in both terms were analyzed stastistically. The most popular reason for withdrawal from the study was "leaving from the institution by a care contact". There was no withdrawal due to the side effects of test foods. We observed symptoms such as "cough", "fatigue", "fever", "sore throat", "sputum", "nasal signs", and "sore breast", and calculated them, based on the juggements of the medical staff. Although thirteen participants (36.1%) experienced at least one symptom in the both terms, the total scores were significantly high value (p<0.05) in the term of R.B. The average duration in which the participants experienced symptoms was 2.6 days in the term of RB whereas 1.2 days in that of HRB, which was not statistically significant. While there are many reports that HRB increases NK cell activity, no significant data was observed in this study because the participants had enough NK cell acitivites from the start.

HRB shortened the duration of the symptoms, reduced the worse and the necessity of symptomatic therapy, and was useful for the reduction of physical burden of acute respiratory tract infection.

Synergistic Role of Arabinoxylan Rice Bran (MGN-3/Biobran) in S. cerevisiae-induced Apoptosis of Monolayer Breast Cancer MCF-7 Cells peer-reviewed            PEER REVIEWED

  • M. Ghoneum and S. Gollapudi (edited by Chris Gutch PhD.) 2005

We have recently demonstrated that breast cancer cell (BCC) lines undergo apoptosis following phagocytosis of S. cerevisiae. Arabinoxylan rice bran extract (MGN-3/Biobran) has been shown to enhance this effect. Since previous data were obtained using cells in suspension, the present study was undertaken to examine monolayer BCC that more closely model cancer cell growth. Monolayers of both breast cancer (MCF-7) and non-tumorgenic breast epithelial (MCF-10A) cells grown on glass coverslips were cultured with heat-killed S. cerevisiae at a ratio of 1:10, respectively. MCF-7 cells phagocytized yeast in a time-dependent manner, 6.9% to 14.3% from 1 h to 4 h, respectively, with a 2-fold increase in the presence of MGN-3. On the other hand, there was virtually no phagocytosis of yeast by MCF-10A cells. Similarly, yeast-induced apoptosis of MCF-7 cells occurred in a time-dependent manner from 11.5% after 1 h to 21. 7% after 4 h, and was enhanced in the presence of MGN-3. These data may have implications in the treatment of breast cancer.

The Life Prolongation and QOL Improvement Effect of Rice Bran Arabinoxylan Derivative (MGN-3, BioBran) for Progressive Cancer

  • Kihachiro Takahara and Kamataro Sano
  • Clinical Pharmacology and Therapy Vol.14/ No.32004
  • lung cancer, liver cancer, uterine carcinoma, breast cancer, prostate cancer, colon cancer, gastric cancer, lymphoma, complementary alternative medicine, natural killer activity, apothanasia effect
  • 205 cancer patients the BioBran group of 96 subjects the control group of 109 subjects (lung cancer, liver cancer, uterine carcinoma, breast cancer, prostate cancer, colon cancer, gastric cancer, lymphoma)
  • 3 g/day 
  • 18 months

Effect of Long-term Administration of Immunomodulatory Food on Cancer Patients Completing Conventional Treatments

  • Hiroshi Tsunekawa
  • Clinical Pharmacology and Therapy Vol.14/ No.32004
  • care report, gastric cancer, colon cancer, breast cancer, rectal cancer, bile duct carcinoma, ovarian cancer, thyroid cancer, lung cancer, cervical cancer, NK activity, tumour marker, leukocyte count, complementary medicine, immunomodulatory food, safety
  • 16 cancer patients (gastric cancer, colon cancer, breast cancer, rectal cancer, bile duct carcinoma, ovarian cancer, thyroid cancer, lung cancer, cervical cancer)
  • 3 g/day
  • 6 months
  • Long- term administration of BioBran caused no subjective or objective adverse effect in cancer patients with decreased immunity. The main clinical observations were the safety and effect of BioBran on the nutritional state of the patients. During the administration period, the leukocyte count and NK cell activity increased, and tumor markers remarkably decreased. (BB015)

Apoptosis of Breast Cancer MCF-7 Cells In Vitro is Induced Specifically by Yeast and Not by Fungal Myceli                                                                                                  PEER REVIEWED

  • M. Ghoneum and S. Gollapudi, (edited by Chris Gutch PhD.), 2006

lt was recently demonstrated that breast cancer cell lines undergo apoptosis following phagocytosis of S. cerevisiae. The present study was undertaken to determine whether other strains of fungi also induce apoptosis in cancer cells. Eight strains of yeast: C. albicans, C. krusei, C. glabrata, C. kefyr, C. neoformans, Y lipolytica, S. cerevisiae and R. rubra, were evaluated at different developmental stages (small yeast cells, large yeast cells and pseudohyphae). In addition, conidia from I four strains of fungal mycelia: Aspergillus, Aspergillus sp., T rubrum and T tonsurans were evaluated. Breast cancer MCF-7 cells in monolayer were cultured with fungi at a ratio of 1:10, respectively, after which phagocytosis and fungi-induced apoptosis of MCF-7 cells were examined. The MCF-7 cells were found to phagocytize all strains of heat-/cilled yeast, regardless of their evelopmental stage. Phagocytosis followed a graduated series: C. krusei > C. glabrata > R. rubra > S. cerevisiae > C. kefyr > C. neoformans > C. albicans > Y. lipolytica. Yeast-induced apoptosis also followed a graded pattern: S. cerevisiae > C. kefyr > C. krusei > C. neofonmans > R. rubra > C. albicans > C. glabrata > Y. lipolytica, as examined by flow cytometry. In contrast, MCF-7 did not phagocytize or undergo apoptosis post-culture with conidia. This data may have clinical implications for the treatment of breast cancer.